The index closed 2.24 points lower after dropping 7.19 points in the previous session.
Trading on the Ho Chi Minh Stock Exchange increased by 41% to VND19.616 trillion (US$775 million).
The VN-30 basket, comprising the 30 largest capped stocks, saw 19 tickers fell.
STB of Ho Chi Minh City-based lender Sacombank saw the biggest drop of 4.8%, followed by MWG of electronics retail chain Mobile World with a 3.1% decline and TPB of private TPBank, down 2.7%.
Ten blue chips gained. HPG of steelmaker Hoa Phat Group went up 2.6%, FPT of tech giant FPT Corporation closed 1.8% higher, and MSN of conglomerate Masan Group rose by 1.7%.
Foreign investors were net seller to the tune of VND944 billion, mainly selling MSN and CMG of tech firm CMC Corporation.
The HNX-Index for stocks on the Hanoi Stock Exchange, home to mid and small caps, fell 0.01%, while the UPCoM-Index for the Unlisted Public Companies Market went up 0.20%.
Globally, Hong Kong stocks led declines in Asia on Monday after Beijing's latest stimulus fell short of investor expectations, overshadowing Wall Street's record highs from Friday and futures pointing to further strength at the reopen, Reuters reported.
Hong Kong's Hang Seng sank 1.9% with a sub-index of mainland Chinese property shares tumbling 3.3%.
Chinese blue chips were more volatile, trading 0.2% higher after earlier dipping 1.6%.
Japan's Nikkei was flat after recovering from small early losses. South Korea's Kospi dropped 0.8%.
Australia's share benchmark declined 0.4%, weighed down by commodity stocks, after oil and industrial metals weakened.