The index closed 12.50 points lower after dropping 6.23 points in the previous session.
Trading on the Ho Chi Minh Stock Exchange increased by 33% to VND15.60 trillion (US$632 million).
The VN-30 basket, comprising the 30 largest capped stocks, saw 24 tickers fell.
SSB of SeABank plunged 6.1%, followed by VRE of retail real estate arm Vincom Retail with a 4.5% drop.
HPG of steelmaker Hoa Phat Group and TCB of the largest private lender Techcombank both declined by 1.8%.
Five blue chips bucked the trend. VJC of budget airline Vietjet closed 1.2% higher and TPB of private TPBank ended with a 1.1% increase.
Foreign investors were net sellers to the tune of VND386 billion, mainly selling FPT of IT giant FPT Corporation and MSN of conglomerate Masan Group.
The HNX-Index for stocks on the Hanoi Stock Exchange, home to mid and small caps, fell 0.76%, while the UPCoM-Index for the Unlisted Public Companies Market went down 0.69%.
Asian stocks inched slightly higher on Tuesday but struggled to sustain an upbeat rally on Wall Street as concerns about a faltering Chinese economy dampened the market mood, Reuters reported.
MSCI's broadest index of Asia-Pacific shares outside Japan was last up a marginal 0.05%, languishing near a one-month low hit in the previous session. Its gains were capped by a slump in Chinese stocks.
China's CSI300 blue-chip index fell to a seven-month trough and last traded 0.17% lower, while the CSI Tourism Index, tumbled to a record low, underscoring the country's anemic consumer demand.