The incident took place in international airspace as the U.S. reconnaissance aircraft carried out "a routine U.S. patrol," a Pentagon statement said.
The incident comes a week after China scrambled fighter jets as a U.S. Navy ship sailed close to a disputed reef in the South China Sea (Vietnam's East Sea).
Another Chinese intercept took place in 2014 when a Chinese fighter pilot few acrobatic maneuvers around a U.S. spy plane.
The intercept is also days before President Barack Obama travels to parts of Asia from May 21-28, which will include a Group of Seven summit in Japan and his first trip to Vietnam.
The Pentagon statement said the Department of Defense was addressing the issue through military and diplomatic channels.
The Chinese embassy in Washington did not immediately respond to a request for comment.
"Over the past year, DoD has seen improvements in PRC actions, flying in a safe and professional manner," the Pentagon statement said, using an acronym for the People's Republic of China.
In 2015, the United States and China announced agreements on a military hotline and rules of behavior to govern air-to-air encounters.
China claims most of the South China Sea (Vietnam's East Sea), through which $5 trillion in ship-borne trade passes every year. The Philippines, Vietnam, Malaysia, Taiwan and Brunei have overlapping claims.
Washington has accused Beijing of militarizing the South China Sea (Vietnam's East Sea) after creating artificial islands while Beijing, in turn, has criticized increased U.S. naval patrols and exercises in Asia.
The Pentagon last month called on China to reaffirm it has no plans to deploy military aircraft in the Spratly Islands after China used a military plane to evacuate sick workers from Fiery Cross.
In April 2001, an intercept of a U.S. spy plane by a Chinese fighter jet resulted in a collision that killed the Chinese pilot and forced the American plane to make an emergency landing at a base on China's Hainan island.
The 24 U.S. air crew members were held for 11 days until Washington apologized for the incident. That encounter soured U.S.-Chinese relations in the early days of President George W. Bush's first administration.
Last month, the Pentagon said that Russia had intercepted a U.S. Air Force aircraft over the Baltic Sea in an "unsafe and unprofessional" way.