The U.S.' Woodhall, who is married to Olympic women's long jump champion Tara Woodhall-Davis, timed 46.36sec to beat world record holder Johannes Floors of Germany, who clocked 46.90sec. Dutchman Olivier Hendriks was third.
After crossing the line, 25-year-old Woodhall ran into the arms of his wife who was trackside, just as she had done to him when she won the Olympic long jump title on Aug. 8.
It was the first Paralympic title of Woodhall's career. He won a bronze medal in this event at the Tokyo Olympics three years ago.
Earlier in the Paris Games he finished sixth in the T64 100m.
The Woodhalls' Instagram account charting their adventures at the Olympics and their life since has more than 580,000 followers.