The index closed 12.8 points lower after gaining 15.4 points in the previous session.
On Friday it closed at the lowest since Feb. 2 at 1,174.85 points.
Trading on the Ho Chi Minh Stock Exchange, on which the index is based, rose 9.5% to VND17.49 trillion ($687 million) Tuesday.
The VN30 basket, comprising the 30 largest capped stocks, saw 24 tickers fell.
BCM of Becamex Investment and Industrial Development plunged the steepest with a 4.2% loss, followed by GVR of Vietnam Rubber Group, down 3.8%.
MSN of conglomerate Masan Group declined by 3.3% and VHM of property giant Vinhomes closed 3% lower.
Six blue chips bucked the trend, led by MWG of electronics retail chain Mobile World with a 2.5% rise.
Foreign investors were net sellers to the tune of VND292 billion, mainly selling VHM and DIG of Development Investment Construction.
The HNX-Index for stocks on the Hanoi Stock Exchange, home to mid and small caps, fell 1.19% while the UPCoM-Index for the Unlisted Public Companies Market dropped 0.58%.