The index closed 13.49 points lower after gaining 1.01 points in the previous session.
Trading on the Ho Chi Minh Stock Exchange increased by 14% to VND15.98 trillion (US$629 million).
The VN-30 basket, comprising the 30 largest capped stocks, saw 24 tickers fell.
STB of Ho Chi Minh City-based lender Sacombank and VHM of property giant Vinhomes both plunged 6.7%.
TPB of private TPBank declined by 3.4% and VIC of private conglomerate Vingroup closed 2.7% lower.
Six blue chips gained, led by VNM of dairy giant Vinamilk with a 1.6% increase.
The HNX-Index for stocks on the Hanoi Stock Exchange, home to mid and small caps, fell 0.80%, while the UPCoM-Index for the Unlisted Public Companies Market went down 0.07%.
Asian shares tracked Wall Street lower on Thursday as uncertainty over the U.S. election outcome kept investors on edge, while the dollar stood tall near its three-month high, supported by elevated Treasury yields, Reuters reported.
Tokyo's Nikkei rose 0.1% but MSCI's broadest index of Asia-Pacific shares outside Japan fell 0.6%, pressured by falls in Chinese shares.
Both Hong Kong's Hang Seng index and China's blue chips dropped 1.2%.