The 41-year-old man and 43-year-old woman were filmed engaging in oral sex aboard the tuk-tuk on Sunday, in an incident that sparked public criticism over damage to Thailand's tourism image, Bangkok Post reported.
Police interrogated the couple on Monday night and both confessed to their act, Nation Thailand reported.
They were charged with public indecency and immigration police said that the couple have been blacklisted and deported.
Public sex and indecent acts are punishable by fines of up to 5,000 baht (US$155) under Thai law.
However, the penalties have done little to deter some tourists from engaging in public nudity and other inappropriate behaviour outdoors.
On April 12, a French couple, both 24, were arrested after allegedly engaging in a public sex act on Rayee Beach. The pair were later deported after videos circulated online showing the nude woman straddling her boyfriend and performing sexual acts in full view of beachgoers.
Thailand, whose economy heavily relies on tourism, has vowed to tighten oversight of tourists' behavior after a series of incidents involving foreigners caught having sex in public, warning they damaged the country's image, AFP reported.