The index closed 7.67 points lower after losing 44.98 points on Tuesday.
Trading on the Ho Chi Minh Stock Exchange (HoSE) fell by 40.32% to VND14.04 trillion ($588.31 million).
The VN-30 basket, comprising the 30 largest capped stocks, saw 15 tickers dropped.
NVL of property developer Novaland Group plunged 6.8% to the floor while VPB of private lender VPBank and GVR of Vietnam Rubber Group dropped 4.1% and 3.8%, respectively.
PLX of fuel distributor Petrolimex lost 3.3%, and HDB of HDBank went down 3%.
Twelve blue chips gained, led by VIC of biggest private conglomerate Vingroup with a 6.9% rise, followed by PDR of Phat Dat Real Estate Development, up 1.9%.
CTG of state-owned lender VietinBank grew 1.3%, and KDH of real estate firm Khang Dien House went up 1.2%.
Foreign investors were net buyers to the tune of VND1 trillion, the 13th net buying session in a row, mainly focusing on VIC and VHM of property giant Vinhomes.
The HNX-Index at the Hanoi Stock Exchange, where mid and small caps list, was down 1.35 percent while the UPCoM-Index at the Unlisted Public Companies Market was down by 0.80 percent.