The index closed 4.25 points lower after losing 15.27 points on Tuesday.
Trading on the Ho Chi Minh Stock Exchange (HoSE) fell by 17.47% to VND14.41 trillion ($607.63 million).
The VN-30 basket, comprising the 30 largest capped stocks, saw 13 tickers fell.
PDR of Phat Dat Real Estate Development and NVL of property developer Novaland Group both hit floor price with a 7% and 6.7% decline, respectively.
KDH of real estate firm Khang Dien House dropped 2.7%, and SSI of leading brokerage SSI Securities Corporation fell 2.5%.
Other decliners included POW of electricity producer Petrovietnam Power Corporation and CTG of state-owned lender VietinBank.
Fourteen blue chips gained, led by STB of Ho Chi Minh City-based lender Sacombank with a 4.9% gain.
VPB of private lender VPBank dropped 2% and VNM of dairy giant Vinamilk fell 1.3%.
Foreign investors were net buyers to the tune of VND1.66 ($70 million), mainly buying EIB of private lender Eximbank and HPG of steelmaker Hoa Phat Group.
The HNX-Index at the Hanoi Stock Exchange, where mid and small caps list, was down 1.48% while the UPCoM-Index at the Unlisted Public Companies Market was down by 0.46%.