The index closed 15.27 points lower after losing 14.08 points on Monday.
Trading on the Ho Chi Minh Stock Exchange (HoSE) increased by 8.87% to VND17.47 trillion ($735.11 million).
The VN-30 basket, comprising the 30 largest capped stocks, saw 23 tickers dropped.
NVL of property developer Novaland Group plunged 6.9% to floor price.
GVR of Vietnam Rubber Group and PDR of Phat Dat Real Estate Development both fell 6.5%.
VRE of retail real estate arm Vincom Retail lost 5.7%, and TCB of the largest private lender Techcombank dropped 5.3%.
Four blue chips gained, led by VNM of dairy giant Vinamilk with a 2.2% rise.
CTG of state-owned lender VietinBank went up 1.6%.
Foreign investors were net buyers to the tune of VND1.85 trillion, highest in 12 sessions.
They mainly bought VPD of Vietnam Power Development and HPG of steelmaker Hoa Phat Group.
The HNX-Index at the Hanoi Stock Exchange, where mid and small caps list, was down 2.22% while the UPCoM-Index at the Unlisted Public Companies Market was down by 1.51%.