The index closed 9.7 points lower after dropping 4.4 points in the previous session.
Trading on the Ho Chi Minh Stock Exchange increased by 31% to VND23.27 trillion (US$940 million).
The VN-30 basket, comprising the 30 largest capped stocks, saw 22 tickers fell.
VHM of property giant Vinhomes plunged the steepest with a 4.1% loss, followed by POW of electricity producer Petrovietnam Power Corporation, down 2.7%.
VRE of retail real estate arm Vincom Retail closed 2.6% lower and TCB of private lender Techcombank dropped 2.2%.
Eight blue chips bucked the trend, led by VPB of private lender VPBank, up 1.5%, and SSB of SeABank, up 1.4%.
Foreign investors were net buyers to the tune of VND575 billion, mainly buying VPB of private lender VPBank and FPT of IT giant FPT Corporation.
The HNX-Index for stocks on the Hanoi Stock Exchange, home to mid and small caps, fell 0.72%, while the UPCoM-Index for the Unlisted Public Companies Market went down 0.64%.
Asian shares retreated from a 32-month peak on Thursday as the sizzling rally in Hong Kong took a breather, while Japan's Nikkei jumped as the risk of further tightening in monetary policy this year faded, Reuters reported.
MSCI's broadest index of Asia-Pacific shares outside Japan fell 1% largely driven by a 1.6% drop in Hong Kong's Hang Seng index.