The index closed 5.50 points lower after dropping 2.24 points in the previous session.
Trading on the Ho Chi Minh Stock Exchange decreased by 28% to VND14.22 trillion (US$561 million).
The VN-30 basket, comprising the 30 largest capped stocks, saw 25 tickers fell.
MWG of electronics retail chain Mobile World dropped 3.5%, followed by GVR of Vietnam Rubber Group with a 1.5% decline.
MSN of conglomerate Masan Group decreased by 1.4% and CTG of state-owned lender VietinBank closed 1.3% lower.
Five blue chips gained, led by SAB of brewer Sabeco with a 2.4% increase.
Foreign investors were net sellers to the tune of VND608 billion, mainly selling TCB of private lender Techcombank and PVD of PetroVietnam Drilling & Well Services Corporation.
The HNX-Index for stocks on the Hanoi Stock Exchange, home to mid and small caps, fell 0.08%, while the UPCoM-Index for the Unlisted Public Companies Market went down 0.01%.
Asian stocks tumbled on Tuesday dragged by Chinese markets and chip shares as investors worried about U.S. President-elect Donald Trump's policies, while bitcoin hit a record peak on bets on assets that are set to benefit from the new administration, Reuters reported.
MSCI's broadest index of Asia-Pacific shares outside Japan down 1.7% to its lowest since Sept. 25, with Taiwan shares sliding over 2% and South Korean stocks 1% lower.