On the eve of the International Day of the Tiger, WWF said it was crucial for governments to identify and close so-called "tiger farms", which are distinct from zoos or breeding centres with a legitimate conservation mission.
Tiger farms have been linked to the highly lucrative and internationally prohibited trade in tiger parts.
The conservation group estimated that there remained 200 tiger farms in Asia, mostly in China, Laos, Vietnam and Thailand.
"Closure of these operations... would significantly boost efforts to save the world’s remaining wild tigers," WWF said in a statement.
The tiger population in farms is about 8,000, more than double the estimated 3,900 living in the wild, WWF said in a statement.
The so-called Tiger Temple in western Thailand was closed in May after Thai wildlife officials discovered dozens of dead cubs inside a freezer.
"The shocking images from the Tiger Temple of tiger cubs frozen and prepared for the illegal trade provide clear evidence of what is really going on behind the scenes at these tiger farms and why they must be closed," said WWF tiger specialist Michael Baltzer.
Some tiger farm operators have insisted their aim is to provide tourists an opportunity to interact with exotic cats.
But WWF said the "incredibly high operating costs" of these farms made it more likely they were involved in black-market trade.
Tiger parts are sometimes used in Asian remedies which are claimed to boost virility or fight disease.
Tiger farms "undermine efforts to protect wild tigers and halt the illegal trade by complicating enforcement activities, and by normalizing and legitimizing the sale of tiger parts and products, which in turn drives up demand," WWF said.
A hastily-organized blanket closure of all tiger farms would however be disastrous for the animals, the organization added.
Tigers living in farm-like captivity have become habituated to human presence and cannot simply be released in the wild, the group said.
It said a tiger resettlement plan needed to be in place before the farms were closed.
At a conference in St. Petersburg in 2010, 13 Asian countries agreed to double the number of tigers living in the wild on the continent by 2022, which is China's next Year of the Tiger.
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